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Schaffner A, Li X, Gomez-Llorente Y, Leandrou E, Memou A, Clemente N, Yao C, Afsari F, Zhi L, Pan N, Morohashi K, Hua X, Zhou MM, Wang C, Zhang H, Chen SG, Elliott CJ, Rideout H, Ubarretxena-Belandia I, Yue Z

Vitamin B12 modulates Parkinson’s disease LRRK2 kinase activity through allosteric regulation and confers neuroprotection.

Cell Res. 2019 Apr;29(4):313-329. doi: 10.1038/s41422-019-0153-8. Epub 2019 Mar 11.

 

Hedayati M, Zarif Yeganeh M, Sheikholeslami S, Afsari F.

Diversity of mutations in the RETproto-oncogene and its oncogenic mechanism in medullary thyroid cancer.

Crit Rev Clin Lab Sci. 2016 Aug;53(4):217-27. doi: 10.3109/10408363.2015.1129529. Epub 2016 Jan 27. Review.

 

James S, Fox J, Afsari F, Lee J, Clough S, Knight C, Ashmore J, Ashton P, Preham O, Hoogduijn M, Ponzoni Rde A, Hancock Y, Coles M, Genever P.

Multiparameter Analysis of Human Bone Marrow Stromal Cells Identifies Distinct Immunomodulatory and  Differentiation-Competent Subtypes. Stem Cell Reports

Stem Cell Reports. 2015 Jun 9;4(6):1004-15. doi: 10.1016/j.stemcr.2015.05.005.

 

Afsari F, Christensen KV, Smith GP, Hentzer M, Nippe OM, Elliott CJ, Wade AR.

Abnormal Visual gain control in a Parkinson’s Disease model. 

Hum Mol Genet. 2014 Sep 1;23(17):4465-78. doi: 10.1093/hmg/ddu159. Epub 2014 Apr 9.

 

Hindle S, Afsari F, Stark M, Middleton CA, Evans GJ, Sweeney ST, Elliott CJ.

Dopaminergic expression of the Parkinsonian gene LRRK2-G2019S leads to non-autonomous visual neurodegeneration, accelerated by increased neural demands for energy. 

Hum Mol Genet. 2013 Jun 1;22(11):2129-40. doi: 10.1093/hmg/ddt061. Epub 2013 Feb 7.

 

Wade AR, Afsari F, Alamri S, Robertson J, Elliott CJH.

Steady state visually-evoked responses in Drosophila models of neurological disease

Journal of Vision 2013, 13 (15), P38-P38

 

James S, Afsari F, Dyson J, Ashmore J, Knight C, Ashton P, Genever P.

Defining Mesenchymal Stem/Stromal Cell Subpopulations With Variable Differentiation and Immunomodulatory Potential

Journal of Bone and Mineral Research 2013, 28 

 

Afsari F. The role of Wnt signalling pathway in mechanotransduction pathway in SV-40 immortalised human human chondrocyte cell lines.

http://hdl.handle.net/1842/28121

 

Rumsby M, Afsari F, Stark M, Hughson E. 

Microfilament and microtubule organization and dynamics in process extension by central glia-4 oligodendrocytes: evidence for microtubule organizing center. 

Glia. 2003 Apr 15;42(2):118-29.

 

Rumsby M, Afsari F, Stark M, Hughson E. 

Process extension by rat CG-4 line progenitor oligodendrocytes: A positively charged surface promotes a bipolar but not differentiated multipolar morphology and is not necessary for cell growth. 

Neurosci, Res, Com, 1998, 23:2 101-109. 

 

Papers in Preparation

 

Afsari F, Millward-Sadler SJ, Salter DM, Goldring M, Nuki G.  Expression of the components of the Wnt signalling pathway and formation of ligand-receptor-coreceptor complex in SV-40

immortalised human chondrocytes.

 

Afsari F, Millward-Sadler SJ, Salter DM, Nuki G.  GSK3β Kinase Assay Methods and Application In Examining its State of Activity in the Associated Signalling Pathways. Due for submission in Nature Methods.

 

Published Abstracts

 

A.R.Wade, Afsari F, Alamri S, Robertson J, Elliott C J H. Steady state visually-evoked responses in Drosophila models of neurological diseases. Journal of Vision January 1, 2013 vol. 13 no. 15 article P38
 

Afsari F, Millward-Sadler SJ, Goldring M, Nuki G. Expression of components of the Wnt signalling pathway in SV-40 transformed human chondrocytes. International J Exp Pathology, 2001; 82(4), A1.

 

Afsari F, Millward-Sadler SJ, Lee HS, Salter DM, Goldring M, Nuki G. Expression of components of the wingless (Wnt) signalling pathway in SV-40 transformed human chondrocytes. British J Rheumatology, 2001; 40 (Supp 1): 10.

 

Afsari F, Millward-Sadler SJ, Zhou Y, Goldring M, Nuki G., Mechanical signal transduction in SV-40 transformed human chondrocytes; The role of the Wingless (Wnt) signalling pathway. Bone 2002; 30: (Supp3): C89.

 

Afsari F, Millward-Sadler SJ, Salter DM, Goldring M, Nuki G., Expression of Wnt pathway components in human chondrocyte cell lines and the influence of mechanical stimulation on the activity of gylycogen synthase kinase 3b (GSK3b). Osteoarthritis and Cartilage 2003; 11 (Supp A): M7.

 

James S, Clough S, Afsari F, Dyson J, Ashmore J, Knight Ch, Ashton P, Coles M and Genever PG. Functional segregation of immunomodulatory and differentiation-competent mesenchymal stromal cell subpopulations. Abstract submitted and accepted in British Orthopaedic Research Society (BORS) and the paper is aimed to be submitted in CELL STEM CELL. 

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James S, Afsari F, Dyson J, Ashmore J, Knight Ch, Ashton P and Genever PG. Defining MSC subpopulations with variable differentiation and immunomodulatory potential. (Submitted and accepted in the American Society for Bone and Mineral Research, ASBMR)

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Invited oral presentations
 

Afsari F. Expression of the component of the Wnt signalling pathway in SV-40 transformed human chondrocytes.

-BSMB (British Society of Matrix Biology). Newcastle, UK

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Afsari F.Expression of Wnt pathway components in human chondrocyte cell lines and the influence of mechanical stimulation on the activity of GSK3b.

-OARSI (Osteoarthritis Research Society International). Berlin, Germany

 

Poster Presentations

 

Increased neural activity and dopaminergic expression of LRRK2-G2019S accelerates visual neurodegeneration

Hindle S, Afsari F, Stark M, Wade A, Sweeney ST, Eliott CJH.

-Parkinson’s UK Research Conference 2012. York,UK

 

 Using Adult Stem Cells to Treat Bone Cancer?

 Afsari F etal Genever P.

-BCRT (Bone Cancer Research Trust). Manchester, UK

 

Canonical Wnt signalling in 3D Mesenchymal Stem Cell

 Saleh A F, Afsari F, Jaques A, Whyte M, Genever PG.

-UKNSCN (UK National Stem Cell Network). York, UK

 

Expression of the component of the Wnt signalling pathway in SV-40 transformed human chondrocytes.

Afsari F, Millward-Sadler SJ, Lee HS, Salter DM, Goldring M, Nuki G

-BSR (British Society of Rheumatology). Edinburgh, UK

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Expression of the component of the Wnt signalling pathway in SV-40 transformed human chondrocytes.

Afsari F, Millward-Sadler SJ, Lee HS, Salter DM, Goldring M, Nuki G

-BSR (British Society of Rheumatology). Brighton, UK

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Expression of the Wnt pathway components in human chondrocyte cell lines and the influence of mechanical stimulation on the activity of GSK3b.

Afsari F, Millward-Sadler SJ, Lee HS, Salter DM, Goldring M, Nuki G.

-IBMS (International Bone and Mineral Society). Davos, Switzerland

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Expression of the Wnt pathway components in human chondrocyte cell lines and the influence of mechanical stimulation on the activity of GSK3b.

Afsari F, Millward-Sadler SJ, Lee HS, Salter DM, Goldring M, Nuki G

-Wnt meeting (Keystone symposium). NewYork, USA

 

Grants and research funding

 

• A Grant submitted to American Heart association  “ The role of noncanonical Wnt payhway in mechanotransduction pathway in Ischemic Cardiomyocytes.”

 

• A fellowship grant submitted to “Women in Science:L’Oreal” UNESCO, on the 15th of March 2013, in order to investigate the “Effect of dopamine in maintenace of dopaminergic neurons in Parkinson’s disease”.

 

• Application submitted for Summer Students for Post-Docs- a new career development scheme on 19th of March 2013, addressing the below question “Does Wnt signalling regulate the survival of dopaminergic neurons via LRRK2 activity?”

 

Project Proposals for Future Grants Applications

 

• Effective collaboration between NMDAR/Calpain, Wnt/Ca2+ and Wnt/β-catenin pathways is essential in normal development and function of human brain and regulation of CAPN10 gene expression.

 

• Effective collaboration between Notch, Ras and NFκB pathways is essential in normal development and function of human brain and regulation of RALGDS gene expression.

 

 

 

 

 

“The joy of discovery is certainly the liveliest that the mind of man can ever feel”